Bioethanol production process. High dry matter hydrolysis. Palm oil. “… resulted in a solid residue enriched in protein … thereby potentially making a high-protein containing feed supplement.”

  • “Palm kernel press cake (PKC) is a residue from palm oil extraction presently only used as a low protein feed supplement.”1
  • “PKC contains 50% fermentable hexose sugars present in the form of glucan and mainly galactomannan.”
  • “This makes PKC an interesting feedstock for processing into bioethanol or in other biorefinery processes.”

Researchers from Forest and Landscape Denmark, University of Copenhagen, Frederiksberg, Denmark; and from Novozymes A/S, Bagsværd, Denmark; have presented an article titled: “Production of Ethanol and Feed by High Dry Matter Hydrolysis and Fermentation of Palm Kernel Press Cake.”

The researchers from Frederiksberg, Denmark, and from Bagsværd, Denmark, have also noted:

  • “Using a combination of mannanase, beta-mannosidase, and cellulases, it was possible without any pretreatment to hydrolyze PKC at solid concentrations of 35% dry matter with mannose yields up to 88% of theoretical.”
  • “Fermentation was tested using Saccharomyces cerevisiae in both a separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) setup.”
  • “The hydrolysates could readily be fermented without addition of nutrients and with average fermentation yields of 0.43 +/- 0.02 g/g based on consumed mannose and glucose.”
  • “Employing SSF, final ethanol concentrations of 70 g/kg was achieved in 216 h, corresponding to an ethanol yield of 70% of theoretical or 200 g ethanol/kg PKC.”
  • “Testing various enzyme mixtures revealed that including cellulases in combination with mannanases significantly improved ethanol yields.”
  • “Processing PKC to ethanol resulted in a solid residue enriched in protein from 17% to 28%, a 70% increase, thereby potentially making a high-protein containing feed supplement.”
(1) Jørgensen H, Sanadi AR, Felby C, Lange NE, Fischer M, Ernst S: Production of Ethanol and Feed by High Dry Matter Hydrolysis and Fermentation of Palm Kernel Press Cake. Appl Biochem Biotechnol. 2009 Oct 24. [Epub ahead of print]







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