- “Simultaneous saccharification and fermentation (SSF) is an interesting option for ethanol production from lignocellulosic materials.”1
Researchers from Department of Chemical Engineering, Lund University, in Lund, Sweden; have presented an article titled: “Controlled feeding of cellulases improves conversion of xylose in simultaneous saccharification and co-fermentation for bioethanol production.”
The researchers from Lund University have also noted:
- “To meet desired overall yields during ethanol production from lignocellulosic materials, it is important to use both hexoses and pentoses.”
- “This can be achieved by co-fermentation of sugars in SSF, so called SSCF (simultaneous saccharification and co-fermentation), using genetically modified yeast strains.”
- “However, high concentration of glucose in the pretreated material makes xylose utilization challenging due to competitive inhibition of sugar transport.”
- “The present work demonstrates a new approach for controlling the glucose release rate from the enzymatic hydrolysis by controlling the addition of enzymes in SSCF using spruce as the raw material.”
- “Enzyme kinetics and yeast sugar uptake rates for a recombinant xylose utilizing strain of Saccharomyces cerevisiae, TMB3400, were determined in a real hydrolyzate medium.”
- “A simplified model for glucose release and uptake was created, to be used as a tool for control of the glucose concentration in a SSCF process.”
- “With help of this model, an SSCF process with efficient co-utilization of glucose and xylose was successfully designed.”
- “The results showed that the total xylose uptake could be increased from 40% to as much as 80% by controlling the enzyme feed.”
